Three kits covering a wide range of dsDNA concentrations. Luminescence was measured with an integration time of 1000 ms per well on a SpectraMax M5 plate. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. The TRITC and FITC labeling process is conducted as in a previous study . The SpectraMax ® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Windows® 8. 100 μl of 2% EB (Sigma-Aldrich, St. For the Evans blue (EB) dye extravasation test, 3,8. Here, we build on this capability and develop an inducible, self‐propagating transposon platform for continuous genome‐wide mutagenesis and the dynamic. Copia stocks a full line of used microplate readers. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. 5. The MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-After this time, cells were stained with crystal violet, washed 3 times and lysed. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. The measured bioluminescence data were expressed as mean ± SE (n = 3) and fitted with one-site binding model using the. The assay was performed on HDL collected. The plate was then incubated at 37°C for 30 min to allow complement mediated lysis of the cells to take place. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 12. Cells stably expressing wild-type or E633K p110β were. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The guide also covers the software features, the specifications, and the accessories of the systems. The average 526/470 ratio for the sample aliquots. The amount of HRP encapsulated in liposome was calculated from the calibration curve of. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment. SpectraMax M5e Multi-Mode Microplate Reader. Major items of equipment include the following: Agilent MR400 400 MHz two-channel PFG NMR. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 9. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 1. For example, microplates containing different samp les can be read using the same orThe 526/470 ratios for the standards were plotted against the standard concentrations. Shop now. Multimode Reader Comparison Chart. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. , a positive control). SpectraMax Injector Cartridge with SmartInject™ Technology. This is a result of the light being attenuated and scattered by the. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. Page 1 of 8 . 1. 0200-7027. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 1. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Select the Driver tab. Following color development, absorbance was measured at 450nm using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA), and BDNF concentrations in samples were calculated using SoftMax Pro software. The fluorescence measurements of cell experiments were conducted with a SpectraMax M5 plate reader. 2 MΩ cm. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Fluorescence Microplate Reader Comparison Chart. 6 for further experiment, so I'm confused which i would choose for OD value determination. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. Standard curves were plotted using SoftMax® Pro Software (Figure 1). 2016 Aug 2; 7(31): 50027–50042. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Reader Documentation Version No. All values reported represent the mean of at least three independent experiments. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. An emission wavelength of 535 nm was used for carboxy-H2DCFDA and 610 nm was used for DHE and MitoSOX ™. For Western blot analysis of apoptosis marker caspase-3, the equivalent total protein from control and SPM. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 01–10 fmol/well (0. EPOCH2 Plate Reader/Spectrophotometer. Top reading is usually more sensitive. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Flow cytometry analysis H22 cells (5 × 105 cells/well) were seeded in 6-well plates and then cells were treated with or without TB-TF (5, 10, 20, 40, 80 μg/mL) for 48 h. 25 pM (0. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 A standard spectrophotometer measures the absorbance of one sample at a time. Cells were then pulsed with 1μCi 3 H-thymidine (Perkin Elmer, Waltham. Prepare a 1:1 solution of Greiss Reagent 1:Greiss Reagent 2. Extreme caution is recommended when you use organic solvents. Published online 2016 Jul 13. Please contact Tatiana to reserve the plate reader time: tnl1@psu. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. DH5α and BL21 (DE3) chemically competent E. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. A solution of phenol red-free MEM containing 1. With optimized reagents, option to operate the. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 0 . smegmatis using a modified pMV261 vector that allowed protein production upon the addition of 0. 2. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The absorbance was measured using a SpectraMax M5 plate reader from Molecular Divices (San Jose, CA, USA). Supersedes 1. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through. The results were normalized to the amount of total protein compared to the sham. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. Help the planet as you cruise along at 5X speed. The dual monochromator optics allow. Samples were read on a Molecular Devices SpectraMax M5 plate reader at 365-nm excitation and 450-nm emission. Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). siRNA oligonucleotides targeted specifically to rat P2X 7 were used in this experiment. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax M4 reader How does the reader perform a self check?. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. The solution was shaken at 37°C and 1200 rpm for 3 days. 1, and the color is formed by the reaction in the microtiter plate. Chirag considers the SpectraMax M5 a multi-purpose reader that. Plate section without PathCheck applied. The Back Panel Figure 1. Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. 10 μL of each SCGal-Ura and SC-Ura culture were then transferred to 1 mL fresh SC-URA and allowed to grow to saturation overnight. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. 1. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Viability Assay reagent (Promega). SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Cultures were grown for 8 h, and then fluorescence was quantified (excitation, 475 nm; emission, 510 nm) using an M5 SpectraMax plate reader (Molecular Devices). Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 5 × 10 5 counts/fmol, thus the suitable enzyme range for bioluminescence measurement is 0. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Inhibition assays at multiple compound. The threshold of detection was calculated as the mean plus three standard deviations of the luminescence from the supernatant of. The reaction was monitored with a SpectraMax M5 plate reader photometer (Molecular Devices GmbH, Biberach a. Menu. Detection modes are available in UV-Vis absorbance, fluorescence, and. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). The name is the same, but the attitude is completely different. At predetermined time points, the CCK-8 reagent was added into representative wells and the plate was incubated at 37°C for 1 h. begin reading microplates with seven mouse clicks. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. 0 mM of NADH per min at pH 8. Table of Contents. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. T-test comparisons of group mean values (+/− SEM) were used to determine significance of genotype. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2 e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. between the affordability of filter-based readers and the . SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. RUO. 1. Microplate automation and detection instruments for walk away operation and high quality results. After MMP9-antibody complex formation, substrate (20 μM for human and mouse assays, 10 uM for rat) was added and fluorescence was monitored in kinetic mode at 37°C on either a SpectraMax M2 or M5. 6 for further experiment, so I'm confused which i would choose for OD value determination. 7°C and the bottom to 36. edu, 814-863-3503. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). 4. -Glo assay solution (Promega) at room temperature and luminescence measured at 1000 ms −1 integration on a SpectroMax M5. 5. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. com 10. 5 and 10 nm were used for excitation and emission, respectively. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. That explains the lower values on the LmaxII384 plate reader. Results were expressed as absorbance units. M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. 0 and room temperature. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. We use Spectramax M5 microplate reader in our lab. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Turn on Plate Reader and wait for it to complete its start-up routine. There is currently no charge for using this plate reader. Features. 1 M glycine-phosphate-citrate buffers with 0. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). We have run the program successfully on macOS High Sierra using the built-in Terminal and Windows 10 using Anaconda Prompt, processing data from a Molecular Devices SpectraMax M5 plate reader. 6-, 12-, 24-, 48-, 96- and 384-well plates. ) and had a resistivity of 18. Published Dec 16, 2020 | Updated Dec 16, 2020. Place the plate in the SpectraMax M5 plate reader immediately, read OD 405 nm every 30s, last 1 h, 37 °C. 9. 7°C (gradient heating design). The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. PDE-lux transcriptional fusion plasmids were constructed as previously described. Models for In. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. RNA-Seq . White or black. Uncontrolled Copy for Training and Reference Purposes Only . The fluorescence was then measured with a fluorescence plate reader (SpectraMax M5; Molecular Devices, Inc. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Circular Dichroism. Always use a plate lid and do not place a plate that contains these materials in the plate chamber for prolonged periods of time. Copia stocks a full line of used microplate readers. Users can choose to process all plates within the magazine or select a specific number. The modular platform allows upgrading to expand functionality as your laboratory workflows change. 9. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. When a feature or capability applies to only certain readers, this. 2. Table of Contents. Linear, Orbital Slow, and Orbital Fast. A reaction volume of 12 µL was placed into each well of 384-well clear bottom black-walled plate that was sealed with an oxygen impermeable membrane. Last lab had ours for years and never had a problem with it. Purified fluorescent protein was diluted 15-fold into 0. The lower . 5 hours after an initial 10-minute incubation period. Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 345 East 24th Street, New York, NY. The plate reader assay was then repeated in SC-URA media with and without. 2. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. 2. Top reading is usually more sensitive. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The supernatants collected in Subheading 3. Description Figure 1. 2. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The antibiotic bacitracin A has been used as an inhibitor of thiol isomerases, including ERp5, in the insulin reductase assay . 7. Luminescence was detected using the Bright-Glo Luciferase Assay System (Promega) on a SpectraMax M5 plate reader (Molecular Devices). U87 and U251 cells were seeded in 96-well plates at 3 × 10 3. Plate geometry 6- to 384-well plates, Petri dishes Dispense precision ≤2% at 50-200 μL Dispense accuracy ±1 μL or 2% Physical Characteristics Power 250 Watts max. 40266 Email: drugdiscoverytech@invitrogen. Evans blue dye in the supernatant was detected at optical density (OD) 620 nm, using a SpectraMax M5 plate-reader (Molecular Devices, Sunnyvale CA). Typically the system can detect absorbance, luminescence, fluorescence,. 1. 1 and slit widths of 2. We use Spectramax M5 microplate reader in our lab. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Read More. Information on how to reserve the plate readerBioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. SpectraTest ABS2 Absorbance Validation Package performance tests. After 2 h of static incubation at room temperature, the OD 630 of the cell-free supernatants (A) and deionized water controls (Ar) was then measured using a plate reader (SpectraMax M5) at room. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. Monocytes were seeded in 24 or 96 well plates at 5 × 10 5 cells/mL and differentiated to macrophages for 7 days in X-VIVO 10 (Lonza) medium supplemented with 1% human serum as previously described . Good condition cosmetically, some signs of use such as the scratches on front of panel. After that, the medium was. This procedure is illustrated in Fig. During the room temperature incubation (step 9. Fluorescence Microplate Reader Comparison Chart. 2. 99 was required for a valid standard curve. 130 Watts max. Turn on Plate Reader and wait for it to complete its start-up routine. The mixed samples were incubated for 5 min at room temperature, and their luminescence intensities were then determined by a SpectraMax M5 plate reader. To update the driver. to meet all your research needs. Figure 9. For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. Turn the reader on and secure the USB cable. PPi. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 3) At the bottom of the strip, click on "Show. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. 1 M NaCl at varying pH levels (3–10) and incubated at room temperature for 1 h. 2. FilterMax F3 and F5 Multi. Spend a little more and you can get an FTDI-based USB cable. easy to use and read multiple plates very quickly. 2. 3) At the bottom of the strip, click on "Show. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Cultures were grown at 30°C and 900 rpm for 24 h, then OD600 was measured on a SpectraMax M5 plate reader to determine bacteriostatic potential. A 4-parameter fit using the SoftMax Pro software included with the M5 Plate Reader was drawn through the data and a correlation coefficient (R 2) value > 0. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 5. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Plate chamber design of the SpectraMax category 1 microplate readers. Fluorescence Microplate Reader Comparison Chart. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. SpectraTest® Validation Plates User Manual & Protocol Download Page. Luminescence values were measured on a SpectraMax M5 plate reader (Molecular Devices). Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Uncontrolled Copy for Training and Reference Purposes Only . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Temperature control range from Ambient +4º to 50ºC. Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. 2. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Microplate Reader Shelf. Caspase activity measurementAfter addition of the 50-fold diluted nanoluciferase substrate (Promega, diluted in phosphate buffered saline, 50 μl/well), the bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular. Plate Shaking. After 30 min of induction, cells were transferred to a clear-bottom black 96-well plate, and both GFP fluorescence and optical density were measured using a SpectraMax M5 plate reader. SA-β-gal assay. Table of Contents. GraphPad Prism was used to process the data and calculate the Km and Vmax values. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). After the plate is read, data will appear in the plate section: 11. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Kinetic measurements were done on a SpectraMax M5 plate reader (Molecular Devices) monitoring absorbance at 380 nm at 22 °C using at least three independent measurements. Category 2 and 3 microplate readers use gradient heating as well. 흡광, 형광, 발광 중 하나의 측정 모드만 이용할 수. com 10. Thank you!. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Cell morphology HeLa & caski cells were, respectively, plated in 6-well plates to logarithmic growth phase. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Nonspecific binding was determined by competition with 1. SpectraTest ABS2 Absorbance Validation Package performance tests. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. The ROS level was expressed in arbitrary units (DCF mg −1 protein). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 3. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. 40266 Email: [email protected]. 1) Select the correct plate and the wells that you want to measure. 7°C (gradient heating design). Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readers Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Kinetic data for Omnia® assays on the SpectraMax® M5/M5e reader. With optimized reagents, option to operate the readers. Bioz Stars score: 91/100, based on 1 PubMed citations. 6 for further experiment, so I'm confused which i would choose for OD value determination. 2 only) SpectraMax M-series Microplate readers. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Supersedes 1. We would like to show you a description here but the site won’t allow us. 9. The reduced readings obtained from the plate reader were analyzed using PRISM 6. In general, reading fluorescence from the top is more sensitive than reading from the bottom. RoHS Compliant. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. plates with an 8-fold dispenser (Bio Tek Germany, Bad Friedrichshall, Germany) and the plates were then centrifuged for 1 min at 1,000 rpm and room temperature. This is a result of the light being attenuated and scattered by the. the contents were carefully mixed by pipetting up and down. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The plate can be shaken (icon right of thermometer, and see automix option in setup). There is currently no charge for using this plate reader. IMAP assays are based on binding of. 5: Schematic of the back panel of a reader. FeCl 3, iron standards are always included (0-4 µg /ml).